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Luteinizing Hormone (LH)
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Luteinizing Hormone (LH)
The Quantitative Determination of Luteinizing Hormone Concentration in Human Serum by a Chemiluminescence Immunoassay
Summary and explanation of the test
Luteinizing hormone (LH) is a glycoprotein consisting of two subunits with a molecular mass of 30000 daltons. The α-subunit is similar to other pituitary hormones follide stimulating hormone (FSH), thyroid stimulating hormone (TSH) and chorionic gonadatropin (CG) while the β-subunit is unique. The β-subunit confers the biological activity to the molecule. The α-subunit consists of 89 amino acid residues while the β-subunit contains 129 amino acids. The carbohydrate content is between 15% and 30%.
The clinical usefulness of the measurement of luteinizing hormone (LH) in ascertaining the homeostasis of fertility regulation via the hypothalamic-pituitary-gonadal axis has been well established, In addition, the advent of in vitro fertilization technology to overcome infertility-associated problems has provided the impetus for rapid improvement in LH assay methodology from the technically demanding bioassay to the procedurally simple and rapid immunoenzymometric assays.
Materials provided for 96-well microplate
1. | Microplate | 1 piece | 96 test/piece |
2. | Calibrators | 6 tubes | 0.5 mL/tube |
Concentrate:0, 2, 6, 20, 60, 200mIU/mL | |||
3. | Label | 1 bottle | 16 mL/bottle |
4. | Substrate A Substrate B | 1 bottle 1 bottle | 6 mL/bottle 6 mL/bottle |
Before using, the substrates A and B are mixed by equal volume in practicality requisite amount. | |||
5. | Washing buffer | 1 bottle | 20 mL/bottle |
Before using, being diluted to 600ml with distilled water | |||
6. | Instruction book | 1 piece | 1 piece/kit |
Keeping in 2~8Degree Celsius , is 12 months
Specimen collection and preparation
The specimens shall be blood serum in type and the usual precautions in the collection of venipuncture samples should be observed. For accurate comparison to established normal values, a fasting morning serum sample should be obtained. The blood should be collected in a plain red-top venipuncture tube with or without gel barrier. Allow the blood to clot. Centrifuge the specimen to separate the serum from the cells. Samples may be refrigerated at 2~8Degree Celsius for a maximum period of five days. If the specimen can not be assayed within this time, the sample may be stored at temperatures of -20Degree Celsius for up to 30 days. Avoid repetitive freezing and thawing.
Test procedure
The kit is in room temperature for more than 15mins, then
Unit:μL
Microplate Blank well Standards well Samples well |
Calibrators — 25 — Specimens — — 25 Label — 150 150 |
Swirl the microplate gently for 2mins to mix and cover, incubate 45 mins at 37Degree Celsius . After washing for 5 times, the substrates are added. |
Substrate A 50 50 50 Substrate B 50 50 50 |
Or we also can mix the substrates in a bottle before using, and then pipette 100μL to each wells. |
Swirl and detect |
Detect After substrates being pipetted, suggest detecting in 1-3 mins by luminometer, one second per well. |
The data of concentrate and RLU is fitted in linear by log(X)-log(Y).
Expected values for the FSH (in mIU/mL)
Females: Follicular Phase: 1.0~10 mIU/mL; Midcycle: 15~70 mIU/mL;
Luteal phase: 0.1~13.2 mIU/mL; Postmenopausal: 12.8~47 mIU/mL;
Menstruation: 0.1~11 mIU/mL
Males: 0.7~7.0 mIU/mL
Performance characteristics
A: Precision
With assay precision is less than 10%, between assay precision is less than 15%.
B: Accuracy
Between 90%-110%
C: Sensitivity
Less than 1mIU/mL
Manufacturing enterprise: Beijing Savant Biotechnology Co., Ltd.
Registration: Beijing Drug Administration(prospective) 2400116, the first word in 2009
Luteinizing Hormone (LH)
